Coding
Part:BBa_K525009:Design
Designed by: Manuel Wittchen Group: iGEM11_Bielefeld-Germany (2011-10-25)
Fusion Protein of S-Layer SgsE and LigA with His-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 167
Illegal BglII site found at 1022
Illegal BglII site found at 3819 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 76
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1657
Illegal SapI.rc site found at 4033
Design Notes
This part was assembled by Freiburg Standard Assembly (RFC 25)
Source
SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.