Coding

Part:BBa_K525009:Design

Designed by: Manuel Wittchen   Group: iGEM11_Bielefeld-Germany   (2011-10-25)


Fusion Protein of S-Layer SgsE and LigA with His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
    Illegal BglII site found at 1022
    Illegal BglII site found at 3819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1657
    Illegal SapI.rc site found at 4033


Design Notes

This part was assembled by Freiburg Standard Assembly (RFC 25)


Source

SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.

References